PLEASE NOTE: This webpage was developed as a component of a course offered at the University of Washington:BIO 533- Ecology of Marine Infectious Diseases

My Favorite Gene!By Ana Elisa Garcia

Biomphalaria glabrataHeat-Shock Protein 70 gene
external image images?q=tbn:ANd9GcSVaaJnAFT9H3z2YgOkq40i3nXFbzWaf5yCrV5ebAERFQFzLuV_
Image source:

Taxonomic Information | Gene Function | Gene sequence | Protein sequence | BLAST | Homologues in other Taxa | Conserved Domains | Primer Design | References

Taxonomic Information

Phylym Mollusca. Class Gastropoda. . . Order Basommatophora. . . . . Family Planorbidae. . . . . . Genus Biomphalaria
Catalogue of Life
NCBI Taxonomy Browser

Information on this organism's genome can be found here
The snail Biomphalaria glabrata serves as first intermediate host for the larval stages of several digenetic trematodes. Mechanisms by which these flatworms successfully infect B. glabrata and host defense strategies are not fully understood yet.

Gene Function

Heat shock proteins (HSPs)are involved in the folding and unfolding of other proteins. Their function is to protect cells from irreversible damage and death when they are exposed to stress factors such as infection, toxins, variation in temperature or inflammation.

Heat shock proteins are named according to their molecular weight. Hsp70 refers to families of heat shock proteins of 70 kilodaltons. Proteins with similar structure have been identified in a range of organisms, including molluscs. This protein serves different purposes:
-It can inhibit inflammatory response when it is found intracellularly.-When extracellular, it enhances inflammatory response.
Adema et al. (2010) found that HSP70 is differentially expressed when Biomphalaria glabrata snails are experimentally infected with the trematode Echinostoma parensei. The transcripted is down regulated 2.166 times.

Gene sequence

*The section that is highlighted in brown is what codes for the gene HSP70
The link to the nucleotide sequence can be found here

Protein sequence


The link to the protein sequence can be found here



Homologues in other Taxa


If I run a BLAST with Schistosoma mansoni, I get this

Conserved Domains


Primer Design


link here


Differential transcriptomic responses of Biomphalaria glabrata (Gastropoda, Mollusca) to bacteria and metazoan parasites, Schistosoma mansoni and Echinostoma paraensei (Digenea, Platyhelminthes).Adema CM, Hanington PC, Lun CM, Rosenberg GH, Aragon AD, Stout BA, Lennard Richard ML, Gross PS, Loker ES.Mol Immunol. 2010 Jan;47(4):849-60. Epub 2009 Dec 3.
Regulation of survival gene hsp70.Silver JT, Noble EG.Cell Stress Chaperones. 2012 Jan;17(1):1-9. Epub 2011 Aug 28. Review.
Larval excretory-secretory products from the parasite Schistosoma mansoni modulate **HSP70**protein expression in defence cells of its snail host, **Biomphalaria** **glabrata**.Zahoor Z, Davies AJ, Kirk RS, Rollinson D, Walker AJ.
Cell Stress Chaperones. 2010 Sep;15(5):639-50. Epub 2010 Feb 25.
**Heat-shock** response in a molluscan cell line: characterization of the response and cloning of an inducible **HSP70** cDNA.
Laursen JR, di Liu H, Wu XJ, Yoshino TP.
J Invertebr Pathol. 1997 Nov;70(3):226-33.
Regulation of HSP70 gene expression during the life cycle of the parasitic helminth Schistosoma mansoni.Neumann S, Ziv E, Lantner F, Schechter I.Eur J Biochem. 1993 Mar 1;212(2):589-96.